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Multi-Level Purification Processes After synthesis, Semaglutide API undergoes multiple purification stages, such as: Preparative High-Performance Liquid Chromatography (HPLC) Lyophilization (freeze-drying) Filtration under sterile conditions Preparative HPLC is particularly essential in removing: Truncated peptides Deletion sequences Oxidized impurities Residual solvents A reliable Semaglutide API manufacturer in India ensures that purity levels typically exceed 98–99%, depending on pharmacopeial requirements
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